Isolation of lactobaciulus

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Como isolar, separar lactobacilos ¿Qué se necesita ?

Agar

MRS agar From Wikipedia

Often abbreviated to MRS, this type of bacterial growth medium is so-named by its inventors: de Man, Rogosa and Sharpe. Developed in 1960, this medium was designed to favour the luxuriant growth of Lactobacilli for lab study. It contains sodium acetate, which suppresses the growth of many competing bacteria (although some other Lactobacillales, like Leuconostoc and Pediococcus, may grow). This medium has a clear brown colour.[1] Typical composition

MRS agar typically contains (w/v):[2]

   1.0 % peptone
   0.8 % egg extract
   0.4 % yeast extract
   2.0 % glucose
   0.5 % sodium acetate trihydrate
   0.1 % polysorbate 80 (also known as Tween 80)
   0.2 % dipotassium hydrogen phosphate
   0.2 % triammonium citrate
   0.02 % magnesium sulfate heptahydrate
   0.005 % manganese sulfate tetrahydrate
   1.0 % agar
   pH adjusted to 6.2 at 25°C

The yeast and meat extracts and peptone provide sources of carbon, nitrogen and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids specifically required by Lactobacilli. Polysorbate 80 is a surfactant which assists in nutrient uptake by Lactobacilli. Magnesium sulfate and manganese sulfate provide cations used in metabolism.

ox bile/oxall

Principles of the Procedure Oxbile is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids.

http://www.neogen.com/Acumedia/pdf/ProdInfo/7216_PI.pdf


http://www.titanbiotechltd.com/specifications/4/ox-bile-powder-dried-bacteriological-grade/425.html

esto valdría http://www.amazon.com/Nutricology-Bile-500-Vegetarian-Capsules/dp/B0002JIUDS


ahhhh creo que esto es lo mejor y más barato esperemos que funcione

http://www.alibaba.com/product-detail/Ox-bile_60362915725.html?spm=a2700.7724838.0.0.qYhIfU

bromocresol purple

Bromocresol purple (BCP) or 5',5"-dibromo-o-cresolsulfophthalein, is a pH indicator. In its sultone form, it has a pKa value of 6.3,[1] and is usually prepared as a 0.04% aqueous solution. Bromocresol purple (pH indicator)

below pH 5.2 above pH 6.8 5.2 ⇌ 6.8

Uses

Imagenes de diferentes especies se lACTOBACILUS

Besides its primary function as an indicator, bromocresol purple is used in medical laboratories to measure albumin, and as an addition to acid stop baths used in photographic processing as an indicator that the bath has reached neutral pH and needs to be replaced.

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http://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-13-189